I’d suggest you just contact a service provide, like the AGRF (http://www.agrf.org.au/services/next-gen-sequencing ) or Ramaciotti Centre (https://www.ramaciotti.unsw.edu.au/sequencing/rna-sequencing ), for a quote. They can help you work out what you need.

A/Prof Kathryn Burdon | Principal Research Fellow | Cancer, Immunology & Genetics Group
Menzies Institute for Medical Research | University of Tasmania
Email: Kathryn.burdon@utas.edu.au

Phone: +61 3 62264288

17 Liverpool St (Private Bag 23)
Hobart TAS 7000 | www.menzies.utas.edu.au

From: bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Peter.Grewe@csiro.au
Sent: Thursday, 11 May 2017 9:35 AM
To: Narissa Bax <narissa.bax@utas.edu.au>
Cc: bioinformatics-list@utas.edu.au; Jac Charlesworth <jac.charlesworth@utas.edu.au>
Subject: Re: ... and if you thought sequencing was all nice and accurate

Oh I think it's quite expensive so for pop genetics maybe low sample sizes

Happy to discuss with you next week when I'm back in Hobart

Cheers

Pete

Sent from my iPhone

On 10 May 2017, at 7:00 pm, Narissa Bax <narissa.bax@utas.edu.au> wrote:

Hi there, 

I was wondering if anyone on this list has done any RNAseq work and has an idea of sample quality and costs? 

If so could you please send me an email - I need help for a grant proposal. 

Kindest regards,

Narissa

Dr. Narissa N. Bax 

Adjunct Researcher

Institute for Marine and Antarctic Studies (IMAS)

Project: www.asccc.co.uk

Mobile: +61 (0) 4 1458 3481

---

From: bioinformatics-list-bounces@list.utas.edu.au <bioinformatics-list-bounces@list.utas.edu.au> on behalf of Jac Charlesworth <jac.charlesworth@utas.edu.au>
Sent: Monday, May 1, 2017 1:42 PM
To: Michael Charleston; bioinformatics-list@utas.edu.au
Subject: RE: ... and if you thought sequencing was all nice and accurate

Thankfully not likely to be an issue for any of us given the slim chance we’d have the $ or throughput to use a HiSeq 4000/ExAmp – but always worth remembering that platform variation is huge and the technology and protocol can have a huge impact on any results!

From: bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Michael Charleston
Sent: Thursday, 27 April 2017 4:22 PM
To: bioinformatics-list@utas.edu.au
Subject: ... and if you thought sequencing was all nice and accurate

https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-distort-results/

Michael Charleston
Associate Professor in Bioinformatics

Co-director of Data, Knowledge and Decisions Research Theme

Head of UTAS node of EMBL-ABR
School of Physical Sciences
University of Tasmania
AUSTRALIA
phone: +61 3 6226 2444

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