https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-dist...
Michael Charleston Associate Professor in Bioinformatics Co-director of Data, Knowledge and Decisions Research Theme Head of UTAS node of EMBL-ABR School of Physical Sciences University of Tasmania AUSTRALIA phone: +61 3 6226 2444
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
Thankfully not likely to be an issue for any of us given the slim chance we'd have the $ or throughput to use a HiSeq 4000/ExAmp - but always worth remembering that platform variation is huge and the technology and protocol can have a huge impact on any results!
From: bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Michael Charleston Sent: Thursday, 27 April 2017 4:22 PM To: bioinformatics-list@utas.edu.au Subject: ... and if you thought sequencing was all nice and accurate
https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-dist...
Michael Charleston Associate Professor in Bioinformatics Co-director of Data, Knowledge and Decisions Research Theme Head of UTAS node of EMBL-ABR School of Physical Sciences University of Tasmania AUSTRALIA phone: +61 3 6226 2444
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
?Hi there,
I was wondering if anyone on this list has done any RNAseq work and has an idea of sample quality and costs?
If so could you please send me an email - I need help for a grant proposal.
Kindest regards,
Narissa
Dr. Narissa N. Bax Adjunct Researcher Institute for Marine and Antarctic Studies (IMAS)
Project: www.asccc.co.ukhttp://www.asccc.co.uk
Mobile: +61 (0) 4 1458 3481
________________________________ From: bioinformatics-list-bounces@list.utas.edu.au bioinformatics-list-bounces@list.utas.edu.au on behalf of Jac Charlesworth jac.charlesworth@utas.edu.au Sent: Monday, May 1, 2017 1:42 PM To: Michael Charleston; bioinformatics-list@utas.edu.au Subject: RE: ... and if you thought sequencing was all nice and accurate
Thankfully not likely to be an issue for any of us given the slim chance we'd have the $ or throughput to use a HiSeq 4000/ExAmp - but always worth remembering that platform variation is huge and the technology and protocol can have a huge impact on any results!
From: bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Michael Charleston Sent: Thursday, 27 April 2017 4:22 PM To: bioinformatics-list@utas.edu.au Subject: ... and if you thought sequencing was all nice and accurate
https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-dist...
Michael Charleston Associate Professor in Bioinformatics Co-director of Data, Knowledge and Decisions Research Theme Head of UTAS node of EMBL-ABR School of Physical Sciences University of Tasmania AUSTRALIA phone: +61 3 6226 2444
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
Oh I think it's quite expensive so for pop genetics maybe low sample sizes Happy to discuss with you next week when I'm back in Hobart Cheers
Pete
Sent from my iPhone
On 10 May 2017, at 7:00 pm, Narissa Bax <narissa.bax@utas.edu.aumailto:narissa.bax@utas.edu.au> wrote:
?Hi there,
I was wondering if anyone on this list has done any RNAseq work and has an idea of sample quality and costs?
If so could you please send me an email - I need help for a grant proposal.
Kindest regards,
Narissa
Dr. Narissa N. Bax Adjunct Researcher Institute for Marine and Antarctic Studies (IMAS)
Project: www.asccc.co.ukhttp://www.asccc.co.uk
Mobile: +61 (0) 4 1458 3481
________________________________ From: bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au <bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au> on behalf of Jac Charlesworth <jac.charlesworth@utas.edu.aumailto:jac.charlesworth@utas.edu.au> Sent: Monday, May 1, 2017 1:42 PM To: Michael Charleston; bioinformatics-list@utas.edu.aumailto:bioinformatics-list@utas.edu.au Subject: RE: ... and if you thought sequencing was all nice and accurate
Thankfully not likely to be an issue for any of us given the slim chance we'd have the $ or throughput to use a HiSeq 4000/ExAmp - but always worth remembering that platform variation is huge and the technology and protocol can have a huge impact on any results!
From: bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Michael Charleston Sent: Thursday, 27 April 2017 4:22 PM To: bioinformatics-list@utas.edu.aumailto:bioinformatics-list@utas.edu.au Subject: ... and if you thought sequencing was all nice and accurate
https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-dist...
Michael Charleston Associate Professor in Bioinformatics Co-director of Data, Knowledge and Decisions Research Theme Head of UTAS node of EMBL-ABR School of Physical Sciences University of Tasmania AUSTRALIA phone: +61 3 6226 2444
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
I’d suggest you just contact a service provide, like the AGRF (http://www.agrf.org.au/services/next-gen-sequencing ) or Ramaciotti Centre (https://www.ramaciotti.unsw.edu.au/sequencing/rna-sequencing ), for a quote. They can help you work out what you need.
A/Prof Kathryn Burdon | Principal Research Fellow | Cancer, Immunology & Genetics Group Menzies Institute for Medical Research | University of Tasmania Email: Kathryn.burdon@utas.edu.aumailto:Kathryn.burdon@utas.edu.au Phone: +61 3 62264288 17 Liverpool St (Private Bag 23) Hobart TAS 7000 | www.menzies.utas.edu.auhttp://www.menzies.utas.edu.au/ [cid:image001.jpg@01CFF754.F8486000]
From: bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Peter.Grewe@csiro.au Sent: Thursday, 11 May 2017 9:35 AM To: Narissa Bax narissa.bax@utas.edu.au Cc: bioinformatics-list@utas.edu.au; Jac Charlesworth jac.charlesworth@utas.edu.au Subject: Re: ... and if you thought sequencing was all nice and accurate
Oh I think it's quite expensive so for pop genetics maybe low sample sizes Happy to discuss with you next week when I'm back in Hobart Cheers
Pete
Sent from my iPhone
On 10 May 2017, at 7:00 pm, Narissa Bax <narissa.bax@utas.edu.aumailto:narissa.bax@utas.edu.au> wrote:
Hi there,
I was wondering if anyone on this list has done any RNAseq work and has an idea of sample quality and costs?
If so could you please send me an email - I need help for a grant proposal.
Kindest regards,
Narissa
Dr. Narissa N. Bax Adjunct Researcher Institute for Marine and Antarctic Studies (IMAS)
Project: www.asccc.co.ukhttp://www.asccc.co.uk
Mobile: +61 (0) 4 1458 3481
________________________________ From: bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au <bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au> on behalf of Jac Charlesworth <jac.charlesworth@utas.edu.aumailto:jac.charlesworth@utas.edu.au> Sent: Monday, May 1, 2017 1:42 PM To: Michael Charleston; bioinformatics-list@utas.edu.aumailto:bioinformatics-list@utas.edu.au Subject: RE: ... and if you thought sequencing was all nice and accurate
Thankfully not likely to be an issue for any of us given the slim chance we’d have the $ or throughput to use a HiSeq 4000/ExAmp – but always worth remembering that platform variation is huge and the technology and protocol can have a huge impact on any results!
From: bioinformatics-list-bounces@list.utas.edu.aumailto:bioinformatics-list-bounces@list.utas.edu.au [mailto:bioinformatics-list-bounces@list.utas.edu.au] On Behalf Of Michael Charleston Sent: Thursday, 27 April 2017 4:22 PM To: bioinformatics-list@utas.edu.aumailto:bioinformatics-list@utas.edu.au Subject: ... and if you thought sequencing was all nice and accurate
https://www.wired.com/2017/04/geneticists-fear-illuminas-sequencers-may-dist...
Michael Charleston Associate Professor in Bioinformatics Co-director of Data, Knowledge and Decisions Research Theme Head of UTAS node of EMBL-ABR School of Physical Sciences University of Tasmania AUSTRALIA phone: +61 3 6226 2444
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
bioinformatics-list@list.utas.edu.au
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Jac Charlesworth -
Kathryn Burdon -
Michael Charleston -
Narissa Bax -
Peter.Grewe＠csiro.au